Pre-clinical study demostrates very high sensitivity and specificity

A pre-clinical study is currently performed in collaboration with the University Hospital of Örebro, Sweden. This study is one of several ongoing pre-clinical collaborations with Scandinavian hospitals.

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Clinical blood samples are collected from patients suspected of having sepsis attending the Infectious Disease ER at the University Hospital of Örebro. In addition to the standard 4 blood culture flasks drawn per patient for routine diagnostics, an extra, 5-10 ml, blood sample are taken for the present study. Of the clinical samples collected so far, 33 samples identified as positive in normal clinical routine diagnostics were selected and are presented here.  Pathogen ID and AST analyses were performed directly from blood in a prototype ASTrID system.



High multiplex, high sensitivity testing

Pathogen identification direct from patient blood sample in ASTrID achieved sensitivity of 96% and specificity of 100%, compared to conventional analysis on positive blood cultures.

The figure shows an example of the signal response reported from a multiplexed assay.

Phenotypic AST with MIC results

The phenotypic AST analysis was performed on clinical isolates spiked in blood (inoculum consistent with 10 CFU/ml) and results compared with broth microdilution according to ISO 20776.

The ASTrID AST results showed 99% essential agreement and 99% categorical agreement. The results are highly correlated with EUCAST guidelines.